离子对色谱检测_第三方检测机构_试验中心「北检（北京）检测技术研究院」

注意：因业务调整，暂不接受个人委托测试，望见谅。

常见问题

离子对色谱检测需要多长时间？

通常7-15个工作日，复杂基质样品或涉及多批次方法学验证的检测周期可能延长至20个工作日。

离子对色谱检测费用大概多少？

根据检测项目数量确定，常规单组分定量分析费用在数百元，涉及全项方法学开发与验证的检测费用需根据实际工时及试剂消耗核算。

离子对色谱流动相配制有何特殊要求？

流动相需精确调节pH值并保证离子对试剂充分溶解，使用前必须经过脱气处理，以防止气泡进入检测器影响基线稳定性。

检测信息

离子对色谱是一种通过在流动相中加入与被测离子电荷相反的离子对试剂，使离子型化合物形成中性离子对缔合物，从而在反相色谱固定相上实现高效保留与分离的色谱分析技术，广泛应用于医药、化工、环境等领域的极性化合物和离子型物质检测。

该检测的主要目的是准确定量复杂基质中难分离的离子型目标物，涉及反相液相色谱分离、紫外吸收检测、质谱联用定性等多种分析化学技术手段，以消除基质干扰并提升检测灵敏度。

检测流程涵盖样品前处理去除蛋白质及颗粒杂质、配制含离子对试剂的流动相并充分脱气、设定色谱柱温箱及梯度洗脱程序、进样分离采集色谱峰、很终通过标准曲线法计算目标物浓度，核心参数包括保留时间、理论塔板数、分离度以及加标回收率。

检测范围

烷基磺酸盐类离子对试剂纯度检测
全氟羧酸型离子对试剂含量分析
四丁基溴化铵离子对试剂测定
十六烷基三甲基溴化铵色谱试剂检测
双(2-乙基己基)磷酸酯萃取剂分析
药物制剂中水溶性维生素定量分析
原料药中有机胺类杂质分离检测
生物样本中氨基酸衍生物测定
核苷酸类药物分离与定量检测
无机阴离子混合物分离测定
过渡金属络离子形态分析
多肽及蛋白质水解产物分析
环境水体中极性除草剂残留检测
工业废水中染料及中间体测定
食品添加剂中有机酸含量分析
化妆品中防腐剂成分定性定量
表面活性剂组分分离与纯度分析
离子液体成分构成与杂质测定
季铵盐类杀菌剂有效成分检测
磺酸类染料中间体结构鉴定
药物活性成分解离常数测定

检测标准（部分）

WJ 2122-1993 水质 2,4-二羟基-1,3,5-三硝基苯(斯蒂芬酸)的测定反相离子对液相色谱法
DIN EN 13368-3:2018-07 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 3: Determination of [S,S]-EDDS by ion pair chromatography
DIN EN 15451:2008-11 Fertilizers - Determination of chelating agents - Determination of iron chelated by EDDHSA by ion pair chromatography
ISO 18329:2004 Milk and milk products — Determination of furosine content — Ion-pair reverse-phase high-performance liquid chromatography method
BS EN 13368-3:2017 Fertilizers. Determination of chelating agents in fertilizers by chromatography-Determination of [S,S]-EDDS by ion pair chromatography
BS EN 15451:2008 Fertilizers. Determination of chelating agents. Determination of iron chelated by EDDHSA by ion pair chromatography
ГОСТ EN 15452-2013 Удобрения. Определение хелатированных агентов. Определение железа, хелатированного о,р-EDDHA, посредством реверсивной фазы HPLC
ГОСТ EN 15451-2013 Удобрения. Определение хелатированных агентов. Определение железа, хелатированного EDDHSA, ион-парной хроматографией
UNE-EN 15451:2008 Fertilizers - Determination of chelating agents - Determination of iron chelated by EDDHSA by ion pair chromatography
EN 15451:2008 Fertilizers - Determination of chelating agents - Determination of iron chelated by EDDHSA by ion pair chromatography
ГОСТ EN 13368-2-2016 Удобрения. Определение хелатообразователей методом ионной хроматографии. Часть 2. Определение железа, хелатированного o,o-EDDHA, o,o-EDDHMA и HBED, методом ионной парной хроматографии
DIN EN 13368-2:2018-03 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
BS ISO 18329:2004 Milk and milk products. Determination of furosine content. Ion-pair reverse-phase high-performance liquid chromatography method
BS 07/30171559 DC BS EN 15451. Fertilizers. Determination of chelating agents. Determination of iron chelated by EDDHSA by ion pair chromatography
EN 13368-3:2017 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 3: Determination of [S,S]-EDDS by ion pair chromatography
UNE-EN 13368-2:2007 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by o,o-EDDHA and o,o-EDDHMA by ion pair chromatography
GB/T 24583.8-2019(英文版) 钒氮合金硅、锰、磷、铝含量的测定电感耦合等离子体原子发射光谱法
UNE-CEN/TR 15106:2005 IN Fertilizers - Determination of chelating agents EDDHA and EDDHMA by ion pair chromatography - Comparison of non-standardized Lucena method with EN 13368-2:2001
EN 13368-2:2017 Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
CEN/TR 15106:2005 Fertilizers - Determination of chelating agents EDDHA and EDDHMA by ion pair chromatography - Comparison of non-standardized Lucena method with EN 13368-2:2001